Extensive laboratory tests demonstrate the superior killing ability of Valiant teat dips. Scientists use lab tests to perfect formulations prior to testing in University herds. These tests also help them evaluate characteristics that are difficult to measure using live animals.

Lab tests are limited in that they cannot demonstrate a product’s ability to prevent infections. However, they are quite useful in determining speed and spectrum of kill, effects of organic matter, and in the case of Valiant teat dips, duration of effective killing ability after activation.

AOAC Germicidal and Detergent Sanitizing Tests
Standardized tests developed by the Association of Analytical Chemists (AOAC) reveal a germicide's ability to kill specific organisms on non-porous surfaces. Researchers challenge germicides with millions of bacteria. Upon neutralizing germicidal action after 15 seconds, they determine the reduction in bacterial numbers, expressed in logs.

Valiant Germicidal Efficacy Testing

AOAC Sanitizing Efficacy Results
Contagious Mastitis Causing Organisms

10% Organic Challenge 15 Seconds Valiant Barrier

AOAC Sanitizing Efficacy Results
Environmental Mastitis Causing Organisms
10% Organic Challenge 15 Seconds Valiant Barrier

Some germicides are inactivated by organic matter. Valiant teat dips, tested 14 days after activation, performed equally as well with and without a 10% milk load. With AOAC testing methods, germicides must achieve at least a 5-log reduction in 30 seconds to be considered effective. The Valiant germicide provided complete kill in 15 seconds.

Porcine Skin Test
To more accurately evaluate microbial activity on a porous surface, researchers test germicides on porcine skin tissue. Skin cracks and crevices challenge germicidal performance. Consequently, kill rates on skin are lower than on non-porous surfaces.

Porcine skin closely resembles teat skin characteristics. Because it can be sterilized, it eliminates contamination problems that arise with excised teat tests.

Valiant Barrier Efficacy on Porcine Skin

As compared to a previously tested chlorine dioxide product, Valiant Barrier outperforms UDDERgold® Plus by 1.0 to over 3.5 logs in porcine skin tests. (As a point of reference, a log reduction of 3 is 10 times more than a log reduction of 2.) Note that UDDERgold® Plus was tested one hour after activation while Valiant Barrier was two weeks post activation.

Dried Barrier Test
In addition to the immediate killing action of chlorine dioxide and the physical protection afforded by the dried barrier, Valiant Barrier's residual germicide continues to destroy mastitis-causing organisms long after teats are dipped.

To test the killing ability of linear alkylbenzene sulfonic acid (LAS), the residual germicide found in all Valiant teat dips, researchers applied agar plates seeded with bacteria to the dried film of Valiant Barrier. Log reductions were determined after a 15-second exposure.

Residual Germicide Efficacy

Valiant Barrier achieved superior reductions against both organisms tested. Therefore, a high level of antimicrobial activity is still present in the dried film of Valiant Barrier teat dip.
The residual germicide in Valiant teat dips kills bacteria even after the dip is dry. The graph demonstrates bacterial reductions after exposure of just 15 seconds to the dried film of Valiant Barrier. A 3-log reduction is the same as 99.9% kill.